Biological Monitoring

SRBC Staff Disturbing Stream Substrate to Collect Macroinvertebrates
SRBC Staff Disturbing Stream Substrate to Collect Macroinvertebrates

Data Collection

Paxton Creek travels from the forested Blue Mountains, through residential and commercial areas and empties to the Susquehanna River in a concrete channel along the Cameron Street Corridor. Due to this variation in habitat, the bugs were collected using a multihabitat approach under the EPA’s Rapid Bioassessment Protocols for use in streams and rivers. At each site, 20 ‘kicks’ (or net collections) were taken to sample all the major habitats in proportional representation within the 100 meter sampling reach. All of the bugs from each sample are sorted in the laboratory and identified to the lowest practical taxon (generally genus or species).

 

   

Benthic Macroinvertebrate samples were taken at 15 sites in the Paxton Creek Watershed:

  • 11 of the project’s ambient monitoring sites
  • 3 of the demonstration sites
  • 1 site in the headwaters of Paxton Creek

Statistical Methods

A number of different metrics are used to analyze the macroinvertebrate data. These metrics evaluate the overall number and diversity of macroinvertebrate taxa present in each sample, the percentage of pollutant sensitive and pollutant tolerant species, and the organic pollution tolerance of each species. Three of the metrics are described at the right:

 

Taxonomic Richness: Total number of taxa in the sample. Number decreases with increasing stress.
  • Values ranged from 40 taxa in the Paxton headwaters to only 12 taxa at the mouth of Paxton Creek
  • The average for all sites was about 17

Shannon Diversity Index: A measure of taxonomic diversity of the community. Number decreases with increasing stress.

  • Values ranged from 0.73 at the mouth of Paxton Creek to 2.34 at the Asylum Run tributary
  • The average for all sites was about 1.5
  • An unimpaired reference site in Powell Creek watershed (comparable to Paxton Creek in drainage size, ecoregions, and subecoregions) had a value of 2.60

EPT Index: Total number of Ephemeroptera (mayflies), Plecoptera (stoneflies), and Tricoptera (caddisflies) taxa present in a sample (Three pollutant sensitive taxa). Number decreases with increasing stress.

  • Values ranged from 0 at the mouth of Paxton Creek to 15 at the Asylum Run tributary site
  • The average for all sites was about 6
Habitat Assessments at 65% of the sites within Paxton Creek Watershed were designated as suboptimal.
Habitat assessments pie chart